Cytotoxicity and cellular uptake of ZnS Mn nanocrystals biofunctionalized with chitosan and aminoacids Journal Article

Author(s): Sajimol Augustine M; Anas A; Das, A.V.; Sreekanth S; Jayalekshmi, S.
Article Title: Cytotoxicity and cellular uptake of ZnS Mn nanocrystals biofunctionalized with chitosan and aminoacids
Alternate Title: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Keywords: Nanocrystals; Cytotoxicity; Chitosan; Aminoacids; Biofunctionalized; Cellular uptake
Journal Title: Unknown
Volume: 136
Issue: PB
Publisher: Kerala Science Congress  
Date Published: 2015
Start Page: 327
End Page: 333
Notes: --- - Highly luminescent, manganese doped, zinc sulphide (ZnS:Mn) nanocrystals biofunctionalized with chitosan and various aminoacids such as l-citrulline, l-lysine, l-arginine, l-serine, l-histidine and glycine were synthesized by chemical capping co-precipitation method at room temperature, which is a simple and cost effective technique. The synthesized nanocrystals were structurally characterized by TEM, XRD, EDXS and FT-IR spectroscopy techniques. They possess high colloidal stability with strong orange red photoluminescence emission at 598 nm. The intensity of orange red emission has been observed to be maximum in l-citrulline capped ZnS:Mn nanocrystals in which the emission at 420 nm is effectively quenched by surface passivation due to capping. Taking into consideration the prospects of these highly luminescent, bio-compatible ZnS:Mn nanocrystals in bio-imaging applications, cytotoxicity studies were conducted to identify the capping combination which would accomplish minimum toxic effects. ZnS:Mn nanocrystals biofunctionalized with chitosan, l-citrulline, glycine, l-artginine, l-serine and l-histidine showed least toxicity up to 10 nM concentrations in mouse fibroblast L929 cells, which further confirms their cytocompatibility. Also the ZnS:Mn nanocrystals biofunctionalized with l-arginine showed maximum uptake in in vitro studies carried out in human embryonic kidney cells, HEK-293T, which shows the significant role of this particular amino acid in fetoplacental nutrition. The present study highlights the suitability of aminoacid conjugated ZnS:Mn nanocrystals, as promising candidates for biomedical applications. - "<p>Export Date: 11 February 2015</p>"
CUSAT Authors